Publisher's Synopsis
This is a practical laboratory guide to current techniques of molecular biology and genetics. It is concerned with the methods for the analysis of viral genes and chromosomes. DNA viruses and RNA viruses, including HIV, are also discussed. Detailed experimental protocols are included for: viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein-Barr virus); DNA viruses - virus/host interactions, viral chromosomes, transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus); human immunodeficiency virus/retroviruses - quantitation of HIV-1 virus stocks and RNA, retrovirus reverse transcription/integration, retro-virus-mediated cell fusion, use as cell lineage markers; RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitus delta virus).
