Representative Procedures in Quantitative Chemical Analysis

Representative Procedures in Quantitative Chemical Analysis discusses procedures in relation to their essential features, underlying principles, and varied applications. In first chapter, a combinative method using HPLC-QTOF-MS and HPLC-UV was first established for the identification and simultaneous quantification of the major constituents whose chemical structures are presented. Second chapter focuses on quantitative proteomic analysis of the HFQ-regulon in sinorhizobium meliloti. In third chapter, we present a detailed study on CDR phenomenology and quantitative analysis of wave-front dynamics that allows us to characterize several properties of the mechanism underlying CDRs. The main aim of fourth chapter is to estimate the binding affinity and adhesion force of two targeting molecules, anti-EGFR monoclonal antibody (mAb LA1) and the peptide GE11 (YHWYGYTPQNVI), with respect to EGFR and to compare these values with those obtained for the ligand, EGF. Fifth chapter aims to quantitative phenotyping-based in vivo chemical screening in a zebrafish model of leukemia stem cell xenotransplantation. Sixth chapter explores on quantitative analyses of force-induced amyloid formation in candida albicans ALS5P. In seventh chapter, tissue-specific chemicals of P. quinquefolium were analyzed by laser microdissection and ultra-high performance liquid chromatography- quadrupole/time-of-flight-mass spectrometry (UHPLC-Q/TOF-MS) to elucidate the distribution pattern of ginsenosides in tissues. Eighth chapter aims to analyze the main constituents using ultra-fast performance liquid chromatography coupled to tandem mass spectrometry (UFLC-MS/MS). Ninth chapter main goal is to evaluate reflectance spectroscopy as a tool for TPH assessment, as compared with three commercial certified laboratories using traditional methods. The primary aim of tenth chapter is to develop a direct and rapid RRLC-MS/MS method for simultaneously quantifying the ten constituents in Shen- Fu decoction, namely, ginsenosides-Rb1, Rb2, Rc, Rd, Rg1, Re and Rf and Aconitum alkaloids including AC, MA and HA. Eleventh chapter presents a theoretical study on quantitative structure-antioxidant activity models of isoflavonoids.