Publisher's Synopsis
This volume contains
protocols specifically designed for studying programmed cell death, and also
discusses recent advances in techniques that span broader areas of biology that
have been recently used or that have potential to be incorporated into cell death
research. The protocols are mostly described in the context of mammalian
systems, but also cover other systems such as plants, Drosophila, and
yeast. Programmed Cell Death: Methods and Protocols is comprised of 20
chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9
describe methods for studying apoptosis associated with various pathologies in
different organs including the lymphoid compartment, intestinal epithelium,
granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniques
for studying apoptosis in non-mammalian systems; Chapters 14-16 cover
biochemical and biophysical methods for studying Bcl-2 family protein dynamics
and protein-protein interactions during apoptosis;and the last four chapters
explore protocols that are useful not only in apoptosis research but in wider
areas of biological research, such as genome editing, inducible transgenes, and
proteomics. Written in the highly successful Methods in Molecular Biology
aeries format, chapters include introductions to their respective topics, lists
of the necessary material and reagents, step-by-step, readily reproducible
laboratory protocol, and tips on troubleshooting and avoiding known pitfalls.
Thorough
and cutting-edge, Programmed Cell Death: Methods and Protocols is a
comprehensive and valuable resource for researchers, ranging from beginner to
expert, in their studies on programmed cell death.
