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PCR Protocols in Molecular Toxicology

PCR Protocols in Molecular Toxicology - CRC Press Methods in the Life Sciences.

Paperback (24 Sep 1997)

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Publisher's Synopsis

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

  • Quantitative reverse transcriptase PCR and methods to examine gene expression
  • Differential display cloning
  • Cloning and library screening by PCR
  • Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
  • Basic, non-PCR based molecular biology methods

    PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
  • Book information

    ISBN: 9780849333446
    Publisher: CRC Press
    Imprint: CRC Press
    Pub date:
    DEWEY: 615.9
    DEWEY edition: 21
    Language: English
    Number of pages: 237
    Weight: 381g
    Height: 235mm
    Width: 156mm
    Spine width: 14mm