Publisher's Synopsis
The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR PCR in detecting DNA and RNA PCR in DNA synthesis and mutagenesis PCR cloning of DNA selection of primers radioactive and nonradioactive labeling quantitation of PCR products by HPLC chromosome assignment PCR mapping of HLA genes sequencing of PCR products genomic footprinting quantitation of tumor gene expression screening of phage libraries SSP-PCR and genome walking subcloning of PCR products.
