Publisher's Synopsis
These volumes are a practical laboratory guide to current techniques of molecular biology and genetics. The focus in on eukaryotic cells, particularly human cells and other eukaryotic model systems. Detailed experimental protocols are provided for gene analysis, transcription analysis, transcription factors, splicing, differential display method, PCR, recombinant proteins, antibody methods, receptors, gene amplification strategies, chromosome analysis, yeast artificial chromosomes (YACs), microcloned DNA markers, somatic cell genetic methods, in situ hybridization, chromosomal proteins and DNA, chromatin reconstitution, telomerase and telomeres, nucleoproteins, DNA damage, disease and development, carcinogen analysis, plasimid DNA transfer, and mouse models.