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Measurement and Analysis of Kinetic Isotope Effects

Measurement and Analysis of Kinetic Isotope Effects - Methods in Enzymology

Hardback (13 Sep 2017)

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Publisher's Synopsis

Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD.

Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions.

Book information

ISBN: 9780128122730
Publisher: Elsevier Science
Imprint: Academic Press
Pub date:
DEWEY: 572.7
DEWEY edition: 23
Language: English
Number of pages: 412
Weight: 1134g
Height: 159mm
Width: 236mm
Spine width: 38mm