Publisher's Synopsis
Excerpt from Laboratory Notes of Technical Methods for the Nervous System
If specimens are not thoroughly hardened in Muller's ?uid, the subsequent permanent preservation in strong alco hol does them considerable injury. The alcohol slowly dis solves out the chrome salts and changes the myelin. In the course of a year or two cholesterin crystals form in the alco hol, and minute cavities and vacuoles appear in the speci mens and they do not stain well. If for any reason it is necessary to examine specimens before they are thoroughly hardened, it is best to keep the material for permanent preservation on the shelves in water containing camphor, or dilute, thirty to forty per cent., solutions of alcohol. In the laboratory even thoroughly hardened material is not kept permanently in strong alcohol. Seventy or eighty per cent.
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