Publisher's Synopsis
This volume brings together the latest technological aspects of biochemistry which involve the understanding of enzyme structure in solution, and the definition of the sites required for enzyme catalysis and control. Each chapter is a self- contained review of methods and results, rather than a mere discussion of methodology, and will be of importance to all scientists, whether researching or working in the biochemical technology industries. The book has been planned as a comprehensive coverage of protein engineering and biotechnology applied to enzymes. The authors, carefully selected by Dr Crabbe, describe the cloning of enzyme genes of cDNA for the expression of protein, and the manipulation of enzyme genes for the achievement of modification of the function or control of enzymes. They illustrate the use of the latest techniques in structure determination and prediction, fermentation, isolation, downstream processing and purification of enzymes, to provide lar
