Publisher's Synopsis
As enzymes are biological catalysts that promote the rate of reactions but are not themselves consumed in the reactions; they may be used repeatedly for as long as they remain active. An immobilized enzyme is an enzyme that is attached to an inert, insoluble material such as calcium alginate. The text Immobilized Enzyme Principles provides a sound basis for the design of enzymatic reactions based on kinetic principles. First chapter focuses on peptide-modified surfaces for enzyme immobilization. Second chapter provides a review of several important factors on affecting enzyme immobilization, including immobilization methods, immobilization carrier materials, and immobilization enzyme loading. In third chapter, an approximate analytical method to solve the non-linear differential equations in an immobilized enzyme film is presented. Fourth chapter introduces a novel strategy for the preparation of acetate modified cross-linked gelatin nanoparticles (CLGNs) which showed a reversible temperature-triggered swelling. In fifth chapter, we report the development, fabrication and characterization of an amperometric-based glucose biosensor with the aim of comparing the effectiveness of chitosan of different molecular weights as a matrix for enzyme immobilization using adsorption and crosslinking techniques, and to study the behavior of these enzyme-chitosan electrodes. The purpose of sixth chapter is to immobilize HRP onto IBN-4 nanoparticles via covalent linkages to facilitate the activation of the prodrug IAA (indole acetic acid) in the tumor microenvironment. In seventh chapter, a novel dual functional polymer, NH2-Alginate, is synthesized through an oxidation-amination-reduction process. The aim of eighth chapter is to use a chitin-lignin material as a novel matrix for immobilization by adsorption of the lipase from Aspergillus niger. Ninth chapter highlights and summarizes various studies that have aimed to improve the biochemical properties of industrially significant enzymes. In tenth chapter, PVA/PA6 composite nanofibrous membranes have been formed by electrospinning. In eleventh chapter, we summarize the recent advances of microchannel reaction technologies especially for enzyme immobilized microreactors. In twelfth chapter, the reuse of papain through the recovery of soluble enzyme by centrifugation or by its immobilization on polysaccharides has been evaluated for S. cerevisiae cells deflocculation from fuel ethanol distilleries. Last chapter introduces basic tenets of classic presumptions of enzyme inhibition, types of enzyme inhibitors, different models of enzyme inhibition, and scientific basis of emerging immobilized enzyme technology in different applications.
