Publisher's Synopsis
This book presents the important concepts and advances on how to successfully use genetic engineering techniques on yeast to obtain human proteins and enzymes - an area of vital importance for the production of human proteins for medical purposes.;It establishes and documents the relationship between yeast genetics, heterologous gene expression and the optimized production of recombinant proteins using yeasts, and provides an extended description of general genetics of important yeasts. the book includes the molecular genetics needed to understand the necessary methods of genetic engineering using expression vectors containing foreign (heterologous) genes. Yeast genetics and genetic engineering are described, and microprocessor controlled fermentation and scale-up with yeast given comprehensive coverage. The use of induction in yeast for production and isolation is presented, together with details of secretion strategies.
