Publisher's Synopsis
Progress in gene transfer technology has established the scientific basis for molecular approaches to human diseases. The first trial of somatic cell gene transfer in the kidney was reported in 1991, and several strategies have been developed during the past five years. Gene delivery into specific renal structures allows for evaluation of in vivo effects of specific molecules on particular functions of the kidney. It will ultimately also be useful for intervention in renal diseases by introducing therapeutically relevant genes into the affected sites. Towards achieving these goals, experimental approaches have utilized liposomes, viral vectors, and cell vector systems. In vivo gene delivery into certain renal structures is feasible using these gene transfer vectors. Germ line manipulation, including generation of 'transgenic' and 'knockout' animals, is also becoming a promising and reliable tool. This publication is the first to review the current status of gene transfer approaches to the kidney. Leading experts in this new research field summarize knowledge to date, consider potential applications, and discuss progress and problems.
