Publisher's Synopsis
Encyclopaedia of Molecular Biotechnology Principles and Applications of Recombinant DNA emphasizes on the application of molecular biology to both basic and applied research in biotechnology. Particular areas of interest include the stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting. Molecular biotechnology is the use of laboratory techniques to study and modify nucleic acids and proteins for applications in areas such as human and animal health, agriculture, and the environment. The text Molecular Biotechnology: Principles and Practices provides comprehensive and up-to-date coverage of key concepts in molecular biotechnology. In first chapter, we create a dynamic and practical course that receives positive evaluation by the students of biological sciences and biomedicine Second chapter deals with expression and purification of cellobiohydrolase gene from Penicillium funiculosum NCL1. In third chapter, we report that we successfully cloned and sequenced a chitinase gene from the ovotestis of Kuroda's sea hare Aplysia kurodai. Fourth chapter focuses on whole-genome molecular haplotyping of single cells. Fifth chapter demonstrates the benefits of redesigning a standard lab-based molecular biology course to create a more effective learning environment. Sixth chapter deals with carotenoids from haloarchaea and their potential in biotechnology. The cloning, characterization and functional analysis of Tetrahymena thermophila's DHFR-TS has been presented in seventh chapter. In eighth chapter, we summarize the current state of knowledge of extremophilic enzymes functioning in high salinity and cold temperatures, focusing on their strategy for function at low water activity. Ninth chapter presents marine microbial biodiversity, bioinformatics and biotechnology (M2B3) standards for
