Publisher's Synopsis
Encyclopaedia of Genetic Engineering Shaping the Material of Life introduces some common genetic engineering techniques and focuses on how to approach different real-life problems using a combination of these key issues. Genetic engineering is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. The text Genetic Engineering: A Primer provides an excellent introduction to the area of genetic engineering of plants and animals. The goal of first chapter is to create PCR enzymes with superior performance, as compared to that of WT Taq polymerase. A genetic replacement system for selection-based engineering of essential proteins has been presented in second chapter. The utility of designing homologous primers for the genetic analysis based on the PCR has been discussed in third chapter. Fourth chapter focuses on development of endothelialspecific single inducible lentiviral vectors for genetic engineering of endothelial progenitor cells. In fifth chapter, the expression of PAM, and consequently the C-terminal amidation of recombinant mAbs, has been reduced by two approaches: gene manipulation using RNA interference (RNAi) and zinc finger nucleases (ZFN). In sixth chapter, we report a comparison of SB100X, a newly developed hyperactive SB transposase, to a previous generation SB11 transposase to achieve stable expression of a CD19-specific chimeric antigen receptor (CAR3) in primary human T cells. Seventh chapter examines the professional and scientific views on the social, ethical and legal issues that impact on genetic information and testing in insurance and employment in Europe. In eighth chapter, we report on the genetic modification of pigs to reduce porcine endogenous retrovirus infection risk in the xenogeneic context. In ninth chapter, we discuss on agrobacterium, an agent causing the plant tumor. The history of oncolytic viruses has been revealed in tenth chapter. The goal of eleventh chapter is to widen use of the ? red-recombineering technology to P. ananatis, a bacterium of interest in the field of metabolic engineering. Last chapter focuses on natural genetic engineering.
