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DNA extraction from slug epithelial tissue

DNA extraction from slug epithelial tissue

Paperback (19 Apr 2021)

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Publisher's Synopsis

The aim of the research was to compare three protocols: Extraction with SDS; Extraction with CTAB and 2β-mercaptoethanol and Extraction with SDS and proteinase K, to determine the most appropriate method to obtain DNA of high molecular weight and purity in slug epithelial tissue: Arion subfuscus and Deroceras reticulatum. DNA concentration and purity were estimated spectrophotometrically (absorbance at 260 nm and Abs260/Abs280 nm ratio). A completely randomized design with six replicates was used. Significant differences (p

Book information

ISBN: 9786203638707
Publisher: KS Omniscriptum Publishing
Imprint: Our Knowledge Publishing
Pub date:
Language: English
Number of pages: 68
Weight: 113g
Height: 229mm
Width: 152mm
Spine width: 4mm