Publisher's Synopsis
This title aims to provide basic instruction in the basic procedures of cell culture for newcomers to the field, including aseptic technique, safety and regulatory issues, equipment and materials, media preparation and sterilization, primary culture, propagated cell lines, characterization and authentication, contamination, cryopreservation, and quantitation. There are also a number of specialized protocols some of which have general interest, e.g. cell cloning, 3D culture, scale-up, STR profiling, and some with a with more limited readership, e.g. culture of some specialized cells.