Publisher's Synopsis
Antisense and ribozymes have a comparatively short but successful history as research tools in gene expression studies, and they are considered to have a high potential as reagents in (gene) therapy of viral infections and cancer. This text is a laboratory companion for researchers, technicians and advanced graduate students in experimental medicine, molecular and cellular biology. It provides information on the potential of antisense and ribozyme techniques, and the advantages and limitations of methodology and the potential pitfalls are critically discussed. Strategies for the best choice of targets and delivery system are presented. This information should enable the experimenter to choose the methodology best suited to achieve the aims he or she is pursuing with any human or other biological system. Detailed descriptions of experimental techniques offer a path to fast and reliable experimental success.;This book should be of interest to all researchers interested in the study of gene expression and its control, experimental medicine (oncology, viral infections, AIDS), cell biology, developmental research, molecular biology.
