Publisher's Synopsis
Excerpt from A Comparison of the "Defibrination" And "Oxalate" Methods of Serum Preparation as Applied to Haemorrhagic Septicaemia and Anthrax Sera Together With Some Analyses of Buffalo and Hill Bull Blood
In the case of hill bulls, the' corpuscles settle 'with extreme difficulty and it is usually necessary to centrifugalise the whole of the blood. This curious difierence which is of course Of great practical importance will be dealt with again later in this paper. The oxalated plasma obtained in the above manner is then clotted by the addition Of 10 cc. Of a 12 per cent. Solution Of calcium chloride to each litre Of plasma. An extremely tough, almost white clot forms in a few minutes. This, on sepa ration from the sides of the vessel, rapidly contracts to a very small bulk, this process being accelerated by the addition of weights. The majority Of the corpuscles having been removed before the clot forms, the ex pressed serum is Of an extremely good appearance, being free from any red colouration, a point Of some importance in this country. When carbolised, moreover, serum prepared in this way exhibits much less precipitation than that obtained by defibrination and in the limited time of observation possible, since this method was begun, does not appear to darken in colour so readily. Finally, it has been found that the yields of serum obtained by the oxalate method are, as a rule, markedly higher than by defibrination. This last factor in itself amply repays the in creased time required for the oxalate method.
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